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Chemopreventive Potential of Aloe vera Against 7,12-Dimethylbenz(a)anthracene— Induced Skin Papillomagenesis in Mice

Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India, falak.geet@rediffmail .com

Mali Ram Saini, PhD

Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India

Pradeep Kumar Goyal, PhD

Radiation and Cancer Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur, India

The present investigation was undertaken to explore the antitumor-promoting activity of Aloe vera on 2-stage skin carcinogenesis, induced by a single topical application of 7,12-dimethylbenz(a)anthracene and promoted by treatment of croton oil for 16 weeks in Swiss albino mice. Oral administration of aloe leaf extract at a dose of 1000 mg/kg body weight/d and aloe gel treatment at a dose of 1 mL/ 9 cm²/mice/d was found to be effective in decreasing the number and size of the papillomas. A significant reduction in tumor incidence (40.00 ± 5.10, 30.00 ± 3.25, and 40.00 ± 4.12 for aloe gel, aloe gel and aloe leaf extract combined, and aloe leaf extract alone, respectively) was observed in animals in the aloe extract— and aloe gel—treated groups compared with 100% tumor incidence in the control group. The cumulative number of papillomas during an observation period of 16 weeks was significantly reduced in the aloe-treated groups (8.0 ± 0.34, 6.00 ± 1.10, and 9.00 ± 1.41 for aloe gel, aloe gel and leaf extract, and aloe leaf extract, respectively) compared with a 36 ± 0.98 cumulative number of papillomas in the control group. The average latent period was significantly increased from 4.9 ± 0.10 weeks in the control group to 6.37 ± 0.12, 6.8 ± 0.25, and 6.2 ± 0.21 weeks in the aloe-treated groups, respectively. The tumor burden and tumor yield were significantly decreased (2.0 ± 0.25, 2.00 ± 0.30, and 2.25 ± 0.2 and 0.8 ± 0.25, 0.6 ± 0.32, and 0.9 ± 0.28, respectively) as compared with the 7,12-dimethylbenz(a)anthracene—treated control group (3.6 ± 0.10 and 3.6 ± 0.19). Furthermore, treatment with aloe gel and/or extract by topical and/or oral administration resulted in a significant increase in the reduced glutathione (P < .05), DNA (P < .001), catalase (P < .05), and protein (P < .001) in the skin of mice. Conversely, lipid peroxidation levels were significantly decreased (P < .001) in the skin of mice.

Key Words: chemoprevention • DMBA • croton oil • Aloe vera • chemical carcinogens

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Integrative Cancer Therapies, Vol. 6, No. 4, 405-412 (2007)
DOI: 10.1177/1534735407309079


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Chaudhary, G. Articles by Pradeep Kumar Goyal Search for Related Content PubMed PubMed Citation Articles by Chaudhary, G. Articles by Pradeep Kumar Goyal, Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 7,12-DIMETHYLBENZ(A)ANTHRACENE Medline Plus Health Information Skin Cancer Social Bookmarking                         What's this?