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Chinese Herbs of Shenghe Powder Reverse Multidrug Resistance of Gastric Carcinoma SGC-7901
Jianhua Wang
Fifth Staff Hospital, General Service Ministry of Chinese PLA, Wenxi, Shanxi Province, China
Yuesheng Xia
Fifth Staff Hospital, General Service Ministry of Chinese PLA, Wenxi, Shanxi Province, China, hsiayuer{at}hotmail.com
Huan Wang
Basic Department, Shanxi College of Traditional Chinese Medicine, Taiyuan, China
Zengxia Hou
Changhai Hospital, Second Military Medical University, Shangha, China
The objective of this study was to investigate the reversal effect of Chinese herbs of Shenghe Powder on the multidrug resistance of the human SGC-7901 gastric carcinoma cell line and vincristine-resistant cell line (SGC-7901/vincristine) and the possible mechanism. SGC-7901 and SGC-7901/ vincristine were cultured in liquid medium RMPI 1640, with the addition of vincristine to the vincristine-resistant line. The reversal effect of Shenghe Powder (using verapamil as control) on the multidrug resistance of SGC-7901/vincristine cells was observed using the 3-4,5-dimethylthiazol-2yl) -2,5-diphenylterazolium bromide method. The expression rate of P-glycoprotein (P-gp), lymphoma/leukemia-2 (Bcl-2), and apoptosis ratio of SGC-7901 and SGC-7901/vincristine with added Shenghe Powder, verapamil, or verapamil plus Shenghe Powder was observed by flow cytometry. Shenghe Powder and verapamil decreased the multidrug resistance of SGC-7901/vincristine. The effect of Shenghe Powder (10 mg/L) was significantly higher than verapamil (P < .05). The intracellular concentration of vincristine was increased by Shenghe Powder and verapamil. The vincristine concentration of SGC-7901/vincristine treated with Shenghe Powder was significantly higher (P < .05). Shenghe Powder reduced the expression level of P-gp and Bcl-2 in SGC-7901/ vincristine and increased the apoptotic percentage of tumor cells; Shenghe Powder had the more significant effect on apoptosis (P < .05). In conclusion, Shenghe Powder increases the intracellular concentration of vincristine, consistent with the down-regulation of the expression of P-gp and Bcl-2. The reversal effect of Shenghe Powder was stronger than that of verapamil.
Key Words: multidrug resistance • Bcl-2 • P-glycoprotein • Chinese herbs
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Integrative Cancer Therapies, Vol. 6, No. 4,
400-404 (2007)
DOI: 10.1177/1534735407309765
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