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Integrative Cancer Therapies
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Phyllanthus amarus Inhibits Cell Growth and Induces Apoptosis in Dalton's Lymphoma Ascites Cells Through Activation of Caspase-3 and Downregulation of Bcl-2

Kuzhuvelil B. Harikumar, PhD

Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India, kbharikumar@gmail .com

Girija Kuttan, Ph.D

Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India

Ramadasan Kuttan, Ph.D.

Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India

The authors found in an earlier study that Phyllanthus amarus extract could significantly inhibit the solid and ascites tumor development in mice induced by Dalton's lymphoma ascites (DLA) cells. In the present study, the apoptotic effects of P.amarus against DLA cells in culture was evaluated. P.amarus produced significant reduction in cell viability as determined by the MTT assay. It also induces the formation of apoptotic bodies with characteristic features like plasma membrane invagination, elongation, fragmentation, and chromatin condensation. P.amarus at concentrations of 100 and 200 µg/mL is shown to induce DNA fragmentation. Gene expression analysis reveals that P.amarus induces the expression of caspase-3 and inhibits the expression of Bcl-2, which is an antiapoptotic protein. So the present study provides some insights into the possible mechanism by which P.amarus brings about apoptosis and growth inhibition in DLA cells.

Key Words: Bcl-2 • caspase-3 • Dalton's lymphoma ascites • p53 • Phyllanthus amarus

This version was published on June 1, 2009

Integrative Cancer Therapies, Vol. 8, No. 2, 190-194 (2009)
DOI: 10.1177/1534735408330713
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