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Cytoprotective Effect of Podophyllum hexandrum Against Gamma Radiation is Mediated via Hemopoietic System Stimulation and Up-Regulation of Heme-oxygenase-1 and the Prosurvival Multidomain Protein Bcl-2

Medicinal and Aromatic Plants Laboratory, Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Road, Delhi-110 054, India; rajesharoradr{at}rediffmail.com

R. Sagar, MSc

Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences (Defence Research and Development Organization), Delhi, India; Acharya Narendra Dev College, University of Delhi, Delhi, India

S. Singh, MSc

R. Kumar, PhD

A. K. Sharma, MSc

J. Prasad, BSc

S. Singh, BSc

M. Gupta, PhD

R. K. Sharma, PhD

Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences (Defence Research and Development Organization), Delhi, India

S. C. Puri, PhD

Regional Research Laboratory, Jammu Tawi, India

B. Krishna, MD

Department of Physiology, Maulana Azad Medical College, Delhi, India

M. S. Siddiqui, PhD

Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, India

S. S. Lahiri, PhD

R. P. Tripathi, MD

Division of Radiation Biology and Radioprotection, Institute of Nuclear Medicine and Allied Sciences (Defence Research and Development Organization), Delhi, India

G. N. Qazi, PhD

Regional Research Laboratory, Jammu Tawi, India

The radioprotective effect of a hydroalcoholic extracted material (REC-2000) from the rhizome of Podophyllum hexandrum was studied in mice exposed to lethal radiation (10 Gy). The extract (REC-2000) was found to restore the hemoglobin content (14.73 ± 0.33) and total leukocyte count (TLC) (4166.66 ± 0.02) in lethally (10 Gy) -irradiated mice on the 15th day in comparison to the radiation control mice. The hemoglobin content of the drug + radiation group was observed to be significantly (21.25%) higher than the radiation control group on the 10th day. Similarly, the TLC was significantly increased (83.33 times) in the drug + radiation group as compared to a radiation (10 Gy) only group on the 10th day. Enhanced expression of heme-oxygenase-1 and Bcl-2 protein observed by Western blotting further supports the observation of hemopoietic recovery in irradiated mice. These findings indicate that the bioactive constituents present in REC-2000 exert the radioprotective effect by modulating the hemopoietic system.

Key Words: Podophyllum hexandrum • Himalayan Mayapple • lethal ionizing radiation • hemopoietic system • radioprotection • heme-oxygenase

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